What is thin-layer chromatography silica gel plate - How do you use the thin-layer chromatography silica gel plate
the first time I listen to the thin-layer chromatography silica gel plate, the brain just think what this is, how to use thin-layer chromatography silica gel plate, there are other names, rubber products factory in shenzhen to take everyone to understand what the thin-layer chromatography silica gel plate as well as how to use thin-layer chromatography silica gel plate.
what is a thin layer of silicon sheet
thin silicon sheet and high-performance thin layer silica gel plate is composed of high-quality thin layer silica gel mixture, suitable for adhesive coating on the glass substrate with prescribed entrance, specific surface and pore distribution.
GB / T1497 - 1988 thin-layer chromatography silica gel plate how to use the
activate silicone rubber plate in 100 ℃ oven for 30 min, cooled and draw a straight line from the bottom 2 cm, with a pencil mark spots position gently, of the distance between two points is 1 - 1. 5cm。
b。 Each thin layer chromatography silica gel plate sample amount is commonly 0. 8μL( As the case may be) , the sample point should be as small as possible. If you need to add, the second and third sampling should be performed in the same point after drying of the sample point. Each sample should be dry after the last point, in order to avoid the sample point excessive proliferation, thus affecting the result of the experiment;
after drying at room temperature, rapid sample of the silicon wafer in advance to join expander in the chromatographic column, sealed chromatographic column, began to layer;
d。 The silica gel plate edge from the TLC plate to remove 2 cm, room temperature after the dry air, with 50 ℃ dry 5 min, after cooling for the second time in the chromatographic column. Recommended product: foam silicone rubber plate.
coating development, after the completion of room temperature dry, choose the appropriate method of color to color of paint, and calculate the RF value.
that is rubber products factory in shenzhen with the thin-layer chromatography silica gel plate, thin layer chromatography silica gel plate, how to use the relevant contents of hope to be of help.
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